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Kanupriya, C.
- Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)
Abstract Views :220 |
PDF Views:107
Authors
B. Padmakar
1,
C. Kanupriya
2,
P. Madhavi Latha
2,
C. Vasugi
2,
M. R. Dinesh
2,
D. Sailaja
3,
C. Aswath
2
Affiliations
1 Center for Biotechnology, JNTU, Hyderabad, Telangana, IN
2 ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru - 560089, Karnataka, IN
3 Department of Biotechnology, GRIET, Hyderabad, Telangana, IN
1 Center for Biotechnology, JNTU, Hyderabad, Telangana, IN
2 ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru - 560089, Karnataka, IN
3 Department of Biotechnology, GRIET, Hyderabad, Telangana, IN
Source
Journal of Horticultural Sciences, Vol 11, No 1 (2016), Pagination: 13-20Abstract
The present research focuses mainly on molecular mining and morphological evaluation of guava genome within a full-sib population and, thereby, mapping of quantitative trait loci related to fruit quality traits, viz., seed strength (hardness/softness) and average fruit weight. Linkage maps were enriched for both parental lines, 'Kamsari' and 'Purple Local' using a set of 60 RAPD markers following the pseudo-testcross strategy on a panel of 94 progeny. A total of 480 scorable markers were identified, of which 131 were specific to 'kamsari' and 28 to 'Purple Local', segregating as test cross markers, and, 321 showing intercross pattern common to both. 'Kamsari' spanned a total length of 1959.1cM with average marker interval distance of 3.93cM, while 'Purple Local' spanned a length of 1537.9cM with average marker interval distance of 3.29cM, by forming 11 linkage groups. Estimated genome length observed was 93.02% and 92.77% in 'Kamsari' and 'Purple Local', respectively. Composite Interval Mapping (CIM) was computed at significance of 0.05 and LOD threshold greater than 3.0, which led to detection of one major QTL for the trait of average fruit weight, and, four QTLs for the trait of seed strength (hardness/softness). Of these, two were major and two minor QTLs. Our study provides molecular mapping information on marker-assisted selection for improvement of guava in a breeding program.Keywords
Composite Interval Mapping, Guava, Linkage Map, Pseudo-Testcross, Quantitative Trait Loci (QTL).References
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- Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers
Abstract Views :246 |
PDF Views:131
Authors
C. Kanupriya
1,
D. Manmohan Kumar
1,
P. Nischita
1,
M. Gayathri
1,
K. V. Ravishankar
1,
P. Sampath Kumar
2
Affiliations
1 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, 560089, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, 560089, Karnataka, IN
1 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, 560089, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, 560089, Karnataka, IN
Source
Journal of Horticultural Sciences, Vol 10, No 2 (2015), Pagination: 125-129Abstract
Pomegranate genotypes have been characterized mainly on the basis of morphological traits; but, these traits are affected to a large extent by environmental and cultivation conditions, resulting in their ambiguous discrimination. Molecular markers are more suited for accurate discrimination of genotypes and cultivars. Sequence-Related Amplified Polymorphism (SRAP) markers were used in the present study to analyze polymorphism among the important pomegranate genotypes grown in India. The total number of bands generated by 30 SRAP primers for 12 genotypes was 1448, with an average of 48.3 bands per primer. Polymorphism varied from 2.7 to 73.9, with an average of 40.95%. Similarity-value based on Jaccard's Coefficient ranged from 0.63 (between cvs. Naina and Amlidana) to 0.95 (between cvs. Kabul Yellow and Jalore Seedless). UPGMA (un-weighted pair group method with arithmetic mean) analysis was performed and a dendrogram was constructed using Jaccard's similarity matrix. The 12 genotypes used grouped into 5 clusters. SRAP markers were found suitable for determining variability among the pomegranate genotypes studied.Keywords
Pomegranate, Molecular Markers, SRAP, Genetic Diversity.References
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